Description
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with PLA2G12A. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody preparation specific for PLA2G12A. The competitive inhibition reaction is launched between with pre-coated PLA2G12A and PLA2G12A in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of PLA2G12A in the samples. The color development is stopped and the intensity of the color is measured